The following QC services are part of iPS reprogramming and NES derivation services.
Luminescence based mycoplasma test
we use MycoAlertTM detection kit to analyze 1mL medium that has not been changed for 24-72 hours. The activity of mycoplasma enzymes react with MycoAlertTM substrate, catalyzing the conversion of ADP to ATP. The ATP is transferred into a light signal via the luciferase enzyme in the kit and then measured.
This service can also be offered upon request.
Bright-field (BF) images
Delivery of images from established iPS or NES cells that show cell-specific characteristics.
Confirmation of cell identity
This service is confirming that reprogrammed iPS clones and established NES cells are genetically identical to donor fibroblast cells. Genomic DNA from iPS and/or NES cells are compared to these fibroblasts using the Promega PowerPlex 21 system (??). Relationship between donors and established cell lines are revealed and possible mixup of samples will be avoided.
FACS (cell identity/pluripotency markers)
Expression of cell-identity markers
This service confirms the expression of cell specific markers for established IPS or NES cells. Data will be delivered as percentage of positive cells in FACS plots.